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Image Search Results
Journal: PLoS ONE
Article Title: Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering
doi: 10.1371/journal.pone.0211598
Figure Lengend Snippet: Regulatory elements and coding sequences are cloned as Level 0 parts, enabling one-step assembly into transcriptional units mediated by BsaI (e.g. using the Plant MoClo Toolkit, ) and subsequently into multigene constructs. Numbers in parentheses represent catalogue number at Addgene. **For simplicity, all cassettes are shown assembled on the forward strand. However, the orientation of any cassette in the final construct can be altered by use of a reverse Level 1 acceptor. ‘+S’ = with stop codon ‘-S’ = no stop codon.
Article Snippet: Constructs were assembled using the
Techniques: Clone Assay, Construct